The present study is to understand the types of biomolecules present in the ethyl acetate extract of one herbal plant Cassia absus. Ethnobotanically this plant is used for ailments such as wound healing, eye diseases, skin diseases, irritable bowel syndrome, renal calculi, hypertension etc. The plants are collected from near the paddy fields at Chenagalpattu, Tamil Nadu, India and the ethyl acetate extracts of the whole plant was subjected to GC MS analysis for due processing. Results: The results indicated the presence of many molecules such as Thiocyanic acid, ethyl ester, Cyclopropanecarboxylic acid, 1-amino-, 2-Ethylbutyl (E)-2-methylbut-2-enoate, 4-(2,4-Dimethylcyclohex-3-enyl)but-3-en-2-one, 5,5,8a-Trimethyl-3,5,6,7,8,8a-hexahydro-2H-chromene, 7-Octadecyne, 2-methyl-, n-Hexadecanoic acid, .beta.-D-Mannothiofuranoside, S-n-octyl-, Z-(13,14-Epoxy)tetradec-11-en-1-ol acetate, 1-Heptatriacotanol, .gamma.-Tocopherol, Stigmasterol, .beta.-Amyrin, .beta.-Sitosterol etc. These molecules among others, which have multiple medicinal roles thus indicating this plant as a very important medicinal herb.