Background:Srikhandasavam is a traditional Ayurvedic herbal formulation known for its potential therapeutic properties, particularly in enhancing overall vitality and digestive health. It is composed of a blend of natural ingredients, including sandalwood, which are fermented to enhance their medicinal potency.Aim:The present study investigates the neuroprotective effects of Srikhandasavam on hippocampal neurons subjected to ethanol-induced neurotoxicity.Methods:The primary focus was to assess cell viability and cytotoxicity in the presence of Srikhandasavam at various concentrations, using hippocampal cell cultures.Results:The cytotoxicity results indicated a significant impact at higher concentrations, with 50.3% cell mortality observed at 200 mu g/mL of Srikhandasavam. Conversely, the MTT assay revealed that Srikhandasavam offered notable protection against ethanol-induced neurotoxicity, with a 71.8% cell viability rate at the same 200 mu g/mL concentration.Conclusion:These findings suggest that Srikhandasavam as a potential neuroprotective agent, capable of mitigating ethanol-induced damage in hippocampal neurons while maintaining cell viability at therapeutic concentrations.