The performance and emissions characters of Diesel engine behavior depend largely on several criteria, fuel injection nozzle plays a vital role in the proper combustion of Diesel engines. This research analyzes the impact of a nozzle hole configuration on the features of a biodiesel-fuelled Diesel engine. Therefore, the causes are dependent on the modification that the nozzle hole was selected from three-hole and five-hole nozzles, while the engine characteristics of the lemongrass biodiesel blend with diesel were examined. Lemongrass biodiesel with 20% blend, LGB B20, has been investigated experimentally with different engine loads with respect to brake power, three-hole, and five-hole nozzles. The experimental investigation showed an improvement in peak i.e. highest heat release rate of 12.5% for three- and five-hole nozzle and brake specific fuel consumption of 15% is increased in single hole nozzle and it is observed it???s diminished in three- and five-holed nozzle. Further, the brake thermal efficiency is increased in the five-hole nozzle in comparison to the three-hole nozzle at full load condition. Furthermore emission characteristics like HC, CO, and smoke are decreased with an increase in the number of nozzles, at the penalty of increase in NOx emissions has been observed.