Silver nanoparticles were synthesized, followed by 50 mL of Ficus hispida ethyl acetate extract was mixed with 100 mL of 4 mM silver nitrate solution, and stirring at room temperature, with a color change from yellow to brown indicating silver ion reduction. Characterization included UV-Vis spectroscopy for surface plasmon resonance, FT-IR for functional groups, XRD for crystallinity, FE-SEM and HR-TEM for morphology, and zeta potential and DLS for particle size and stability. The anticancer activity against MCF-7 cells was assessed via the MTT assay, and ROS were detected via fluorescence microscopy. The synthesized FH-Ag-NPs presented a distinct UV-Vis absorption peak at 191.69 nm, confirming nanoparticle formation. FT-IR analysis revealed characteristic peaks at 3,423 cm-1 (O-H stretching), 2,924 cm-1 (C-H stretching), and 1,636 cm-1 (C=O stretching), indicating the involvement of biomolecules in nanoparticle stabilization. The zeta potential value of-31.0 mV suggested good colloidal stability. The DLS results revealed an average particle size of 122.3 nm with a polydispersity index of 0.368, reflecting moderate size uniformity. The XRD patterns confirmed the crystalline nature, with peaks at 10.67 degrees, 27.65 degrees, and 28.18 degrees. Electron microscopy revealed that the spherical nanoparticles were 64-100 nm in size with minimal aggregation. EDAX confirmed that elemental silver was the main component. The FH-Ag-NPs exhibited dose-dependent cytotoxicity to MCF-7 cells, reducing viability from 85.73% (5 mu g/mL) to 42.95% (25 mu g/mL). Fluorescence microscopy revealed increased reactive oxygen species generation at higher concentrations, indicating oxidative stress-induced cytotoxicity. These results confirm the successful green synthesis and the FH-Ag-NPs promising anticancer potential.