In the present investigation, Achromobacter denitrifacians was isolated from industrial wastewater and used in the degradation of para nitro-phenol. Experiments were made as a function of different carbon sources, organic and inorganic nitrogen sources and metal ions to analyse the removal efficiency of para nitro-phenol present in the industrial wastewater sources. Observations revealed that the rate of phenol biodegradation was significantly affected by pH, temperature of incubation, glucose, peptone and metal ion concentration. The optimal conditions for phenol removal was found to be pH of 7.5, temperature, 35 degrees C and 0.25 gL(-1) supplemented glucose level, 0.25 gL(-1) supplemented peptone level, and 0.01 gL(-1) zinc ion. The key importance of the present study is the utilization of native bacterial strain isolated from the industrial effluent water itself having an impending role in the bioremediation process of phenol.