Candidal vulvovaginitis is accountable for a third of all cases of vulvovaginitis in reproductive-aged women, and 70% of women report having or had candidal vulvovaginitis at certain point in their lifespans. Vulvovaginal Candidiasis (VVC) is a recurrent, multifaceted and unwieldy illness that can cause corporeal and mental distress to the individual. Candida albicans was stated as the greatest common cause of VVC yet it appears that we are newly facing changes in the configuration of Candida species in VVC. In the present study we measured diverse species of Candida isolated from patients with VVC. This research was a descriptive analytical cross-sectional study. Candida Sps were isolated from females aged 20 - 60 years, who existing erythema and itching of vulva, vagina, or both and unpleasant vaginal exoneration. Biofilm formation of the isolates were evaluated using crystal violet staining and their drug resistance pattern were evaluated using standard antifungal antibiotic discs. Biofilm production could act as one of the factors in reducing the penetrability of the antifungal agents and also increasing the virulence nature of invasive candidiasis. Biofilm development test was done on all the 85 samples. All sample was prepared in triplicate and the average was determined. Light microscopic biofilm imaging showed the biofilm formation of the budding yeast cells from tiny micro-colonies (2-4 h). After four hours, the budding cells started to divide, and formed pseudo hyphae and ultimately true hyphae. We report that this extremely contagious yeast has the capability to form antifungal resistant biofilms sensitive to the antifungal agent in vitro.