Recent advances in homeopathic medicines have revolutionized the diagnosis and treatment for various health care diseases including severe acute respiratory syndrome. In UV-Vis spectroscopy, the amount of light absorbed is related to the amount of absorbing species present and hence, qualitative information about the medicinal solute species present in Arsenicum album can be obtained. The purpose of this primary research is to elucidate Whether the medicinal solute species exist at higher potencies where the therapeutic effect is potentially high. We have investigated different potencies of Arsenicum album procured from three manufacturers through measurements of optical absorption spectrum using a sophisticated computer controlled UV-Visible 2600 spectrometer. In order to interpret the results, a mother tincture Q sample was used to study the characteristic UV-Vis absorption behaviour of Arsenicum album at various concentrations. The results revealed that irrespective of the potencies, arsenic trioxide medicinal solute species present in the Arsenicum album medicines is in the form of quantum sized nanoclusters. The specific interaction between arsenic trioxide solute species in the Arsenicum album is expected to be reduced upon dilution. Hence, the study of the dilution series of Arsenicum album provides insights. The optical absorption results indicate that arsenic trioxide solutes dilute uniformly in the solvent medium and follow the laws of homeopathy. The study suggests that qualitative analysis of the Homeopathic medicines is possible by UV-Visible spectroscopy.