Background: Chronic kidney disease (CKD) is a gradual loss of kidney function and has an increased prevalence rate worldwide. Our study was intended to identify potential biomarkers of progression using urine proteomics. Materials and Methods: This preliminary study consisted of 32 patients with stage V CKD. Urine samples were subjected to liquid chromatography-mass spectrometry (LCMS), and the network of protein interaction was analyzed using STRING. Results: A total of 135 proteins were identified, of which 35 were listed as candidates based on their clinical significance. Protein- protein interaction study provides novel insights into the functional constitution of the proteome, selecting urine as a source of biomarkers. Conclusion: The present study observed that the potential markers and its progression to CKD stage V.