BACKGROUND Pityriasis versicolor is a superficial cutaneous fungal infection caused by Malassezia furfur, a dimorphic fungus. This study is undertaken to know various clinical patterns of disease, to culture and study the in vitro susceptibilities of Malassezia species to various antifungals. MATERIALS AND METHODS A cross sectional study of 100 patients with Pityriasis versicolor were evaluated clinically at a tertiary care hospital in Puducherry. The study period was 2 years. History and clinical examination was done and scraping samples were sent to Microbiology department for culture and sensitivity. Data was recorded accordingly. RESULTS Majority of the patients were young males. Most of the patients had asymptomatic hypopigmented macules over face and neck. Most of the patients use synthetic clothing and shown exacerbation during summer months. Recurrence is rare. Majority of patients show mild to moderate scaling. Seborrheic dermatitis and acne vulgaris are the most common associated dermatoses. Majority were KOH and Woods lamp positive. Sixty-seven patients were culture positive and most of patients show M. furfur colonies and all patients are sensitive to the three common antifungals. CONCLUSION Overall, our study shows M. furfur is the most common isolated species and all are sensitive to the three commonly used antifungals: Fluconazole, Itraconazole and Ketoconazole. Hence, Malassezia species has not developed resistance against these drugs in our study area, and they can be used successfully in treatment of Pityriasis versicolor.