BACKGROUND Enterococci are Gram-positive cocci that are normal inhabitants of the gastrointestinal tract. Enterococci have become the second most common agent recovered from nosocomial UTI and wound infections and the third leading cause of bacteraemia. Although, enterococci have been considered of relatively low virulence, these organisms can cause serious infections including endocarditis. In the last few decades, the number of serious infections caused by these organisms has been steadily increasing. Resistance to several commonly used antibiotics is a remarkable characteristic of most of the enterococcal species. We aimed at determining the isolation rate and resistance pattern of Enterococcal species to different antibiotics from clinical specimens. MATERIAL AND METHODS A total of 107 Enterococcal species were isolated and identified from different clinical samples by standard microbiological tests. Antimicrobial susceptibility testing was performed by Kirby-Bauer's disc diffusion method as per CLSI guidelines. RESULTS Enterococcus faecalis 85 (79.44%) were predominantly isolated; 22 strains of Enterococcus faecium were isolated and accounted for 20.56%. Majority of isolates were from urine 87 (81.31%) followed by pus 14 (13.08%). High degree of resistance was observed towards penicillin and ampicillin accounting for 94.39%. High level of drug resistance was observed towards gentamicin (64.49%) followed by streptomycin (58.88%). All isolates were found to be susceptible to linezolid and vancomycin. CONCLUSION Enterococcus faecium is comparatively more resistant than Enterococcus faecalis. Identification of Enterococci up to species level may help the clinician to choose the appropriate therapy. Antimicrobial surveillance should be done periodically to monitor the current susceptibility patterns in local hospitals.