Lengthy urinary tract catheterization leading to the bacterial colonization of the urinary tract is a significant determinant of colonization through adherence to the catheter surface. The collection of urine and urinary catheter bacterial isolates of 55 patients was shown to be multiple catheter colonisation, while the Escherichia coli isolates were usually contained in high number in the urine. Kirby Bauer disc diffusion system was used to conduct antimicrobial susceptible patterns of the isolated species. Based on the morphological characterization and some specific biochemical reactions of the most resistant, intermediate and sensitive bacteria, the isolates were identified as, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Candida albicans, Enterococcus Sps., Staphylococcus epidermidis, Proteus mirabilis, and Klebsiella pneumoniae. Nearly 67% the isolates were high biofilm producers with strong adherence.