Selenium nanoparticles (SeNPs) have gained wide importance in the scientific community and have emerged as an optimistic therapeutic carrier agent for targeted drug delivery. In the present study, the effectiveness of nano selenium conjugated with Morin (Ba-SeNp-Mo) produced from endophytic bacteria Bacillus endophyticus reported in our earlier research was tested against various Gram-positive, Gram-negative bacterial pathogens and fungal pathogens that showed good zone of inhibition against all selected pathogens. Antioxidant activities of these NPs were studied by 1, 1-diphenyl-2- picrylhydrazyl (DPPH), 2,2'-Azino-bis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS), hydrogen peroxide -(H2O2), superoxide -(O-2(-)), and nitric oxide (NO) radical scavenging assays that exhibited dose-dependent free radical scavenging activity with -IC50 values 6.92 +/- 1.0, 16.85 +/- 1.39, 31.60 +/- 1.36, 18.87 +/- 1.46, and 6.95 +/- 1.27 mu g/mL. The efficiency of DNA cleavage and thrombolytic activity of Ba- SeNp-Mo were also studied. The antiproliferative effect of Ba-SeNp-Mo was determined by 3-(4,5-dimethylthiazol2- yl)-2,5-diphenyltetrazolium bromide (MTT) assay in COLON-26 cell lines that resulted in -IC50 value of 63.11 mu g/mL. Further increased intracellular reactive oxygen species (ROS) levels up to 2.03 and significant early, late and necrotic cells were also observed in AO/EtBr assay. CASPASE 3 expression was upregulated to 1.22 (40 mu g/mL) and 1.85 (80 mu g/mL) fold. Thus, the current investigation suggested that the Ba-SeNp-Mo has offered remarkable pharmacological activity.