The genus Jacaranda is an important representative of tribe Tecomeae in family Bignoniaceae, with the species native to South America and widely distributed across the tropical regions. This study was aimed to comprehensively screen the phytochemicals of crude extract of Jacaranda mimosifolia and evaluate its antioxidant, anti-inflammatory, and antimicrobial activities. Phytochemical analysis revealed the presence of various bioactive compounds, including glycosides, carbohydrates, tannins, proteins, amino acids, terpenoids, fats, and oils. Antioxidant activity was assessed using ABTS and DPPH scavenging assays, which yielded IC50 values of 144.231 and 12.957 mu g mu L-1, respectively. The extract showed significant antimicrobial activity and effectively inhibited Pseudomonas aeruginosa, Salmonella typhi, Streptococcus mutans and Escherichia coli at various concentrations. The anti-inflammatory potential was evaluated through bovine serum albumin denaturation assay, which showed an IC50 value of 213.85 mu g mu L-1. Additionally, bioinformatics analysis of ribosomal protein L23 in J. mimosifolia revealed structural and physiochemical characteristics associated with its antioxidant properties. The study suggests that J. mimosifolia seed extract could be a promising natural alternative to synthetic drugs for antioxidant, antimicrobial, and anti-inflammatory applications.