The present study evaluated the tyrosinase inhibition activity and antioxidant activity for Vitiligo (pale white patches) disease by using Barleria prionitis (root) extracts with solvent extracts of ethyl acetate. The tyrosinase inhibition activity of the extracts was determined by inhibition concentration (IC50 values). Results showed that ethyl acetate had the highest tyrosinase inhibition activity by decreasing the percentage of inhibition concentration. The extracts' antioxidant activities were assessed by scavenging 1,1diphenyl-2- picrylhydrazyl radicals (DPPH test). The results showed that ethyl acetate root extract of Barleria prionitis has a higher antioxidant activity which proved that it strongly helps to tyrosinase inhibition activity. The findings indicate that extract from ethyl acetate has the strongest tyrosinase inhibitory activity which helps to increase melanin pigmentation in Vitiligo disease affected patients.