The science of life - Ayurveda is practiced in India since time immemorial. Besides being cheap and easily available Ayurvedic drugs are considered safe. Moreover, there is surge in the interest in Ayurveda due to quest of alternative medicines. In Ayurvedic system of medicine, Polyherbal formulations were frequently used to enhance the activity or counteract the toxic effect of compounds, from other plants but may also act synergistically with other constituents from the same plants. Polyherbal formulations (PHF) consist of seven known herbs namely, Aegle marmelos, Elettaria cardamomum, Glycyrrhiza glabra, Citrus aurantifolia, Rosa damascena, Cissus quadrangularis and Saccharum officinarum. The gastrointestinal prokinetics effect of PHF in various dose levels (100mg, 200mg and 400 mg/kg) was studied by charcoal meal gastrointestinal transit and laxative effect in mice. Carbachol (1 mg/ kg) and Sennoside B (50 mg/kg) were used as reference drug. The test drugs were administered orally by suspending in 1% carboxy methyl cellulose solution. The results illustrate, PHF at 200mg and 400 mg/kg significantly (p<0.001) enhanced the gastrointestinal transit. PHF at 400 mg/kg (p<0.01) significantly enhanced the purging index which is the measure of laxative activity. PHF at 200 mg/kg (p<0.05) less significantly enhanced the purging index. From the above findings we conclude that PHF may be used as gastrointestinal prokinetics. © 2008 Elsevier B.V., All rights reserved.