This paper highlights the process of germination of seeds of Coriandrum sativum (CS), using various sterilizing agents and their influence on the physiology of seed. The three sterilizing agents, mercuric chloride (HgCl<inf>2</inf>), sodium hypochlorite (NaOCl) and ethanol (CH<inf>2</inf>OH) were used in the experiment alone and also in combination with each other in various concentrations. The % of seed germination, degree of contamination and seed color during the course of experiment was studied. The aim of this study is to establish best surface sterilization for in-vitro propagation of CS. The seeds of CS were tried to germinate aseptically under various conditions and finally it was observed that seeds showed good germination using sodium hypochlorite (NaOCl) at concentration of 0.15% for 5 minutes. © 2021 Elsevier B.V., All rights reserved.