The current study explores the immunomodulatory activity of Vitex negundo (VN) Linn (verbenaceae), known as Nirgudi which is a medicinally important plant having wide variety of phytoconstituents having significant pharmacological activities. Oral administration of extracts of Nirgudi stem and leaves (100 mg/kg) showed a significant increase (P < 0.05) in body weight of wistar albino rats-experimental animals. A significant increase in the lymphocyte and total leukocyte count as well as in the percent neutrophil adhesion to nylon fibers was observed. In VN treated rats, the phagocytic index was enhanced. The results led to a conclusion that VN possess potential immunomodulatory activity and has therapeutic potential against auto-immune disorders. © 2021 Elsevier B.V., All rights reserved.