This retrospective study identified organisms and antibiotic sensitivity patterns in children with Urinary Tract Infections (UTIs) at a tertiary care hospital in Salem, Tamil Nadu. The sample included 150 children aged 0-14 years. Female children (57.3%) were most affected, with the highest incidence in 1 month–2 years age group (45.33%). E. coli was the most prevalent organism (74.66%). Common symptoms included fever with urinary complaints (35%). Amikacin was the most administered antibiotic (25.33%), and 68% received a single antibiotic. Intravenous administration was most common (76%), and 71% of children were hospitalized for 4–6 days. E. coli showed high sensitivity to aminoglycosides (amikacin, gentamicin) and cephalosporins (ceftriaxone, cefotaxime). © 2020 Elsevier B.V., All rights reserved.